Formate hydrogen lyase mediates stationary-phase deacidification and increases survival during sugar fermentation in acetoin-producing enterobacteria
نویسندگان
چکیده
منابع مشابه
Formate hydrogen lyase mediates stationary-phase deacidification and increases survival during sugar fermentation in acetoin-producing enterobacteria
Two fermentation types exist in the Enterobacteriaceae family. Mixed-acid fermenters produce substantial amounts of lactate, formate, acetate, and succinate, resulting in lethal medium acidification. On the other hand, 2,3-butanediol fermenters switch to the production of the neutral compounds acetoin and 2,3-butanediol and even deacidify the environment after an initial acidification phase, th...
متن کاملHydrogen production during fermentation of acetoin and acetylene by Pelobacter acetylenicus
Pelobacter aceO,lenicus accumulated only small amounts of H, (< 3.5 kPa) during fermentation of acetoin or acetylene to acetate and ethanol. Formate was also produced in small amounts ( -, 0.5 raM). Growth on acetoin was retarded by addition of ethanol, but not by addition of H., or formate. However. addition of H, and/or formate resulted in increased production of 2.3-butanediol. whereas addit...
متن کاملEnhanced hydrogen production from formic acid by formate hydrogen lyase-overexpressing Escherichia coli strains.
Genetic recombination of Escherichia coli in conjunction with process manipulation was employed to elevate the efficiency of hydrogen production in the resultant strain SR13 2 orders of magnitude above that of conventional methods. The formate hydrogen lyase (FHL)-overexpressing strain SR13 was constructed by combining FHL repressor (hycA) inactivation with FHL activator (fhlA) overexpression. ...
متن کاملThe glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation.
Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a PFL but nonetheless was able to complement the phenotypic defects caused by an...
متن کاملScreening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production
Background: Alginate is a linear polysaccharide consisting of guluronate (polyG) and mannuronate (polyM) subunits. Methods: In the initial screening of alginate-degrading bacteria from soil, 10 isolates were able to grow on minimal medium containing alginate. The optimization of cell growth and alginate lyase (algL) production was carried out by the addition of 0.8% alginate and 0.2-0.3 M NaCl ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2015
ISSN: 1664-302X
DOI: 10.3389/fmicb.2015.00150